Cbs-scientific QNX-700 Manuel d'utilisateur télécharger pdf (Page 20)

Quadra Instructions 9-30-13

6.4 Recommended ClearPAGE™ Buffer Formulations, con’t.

LDS Sample Buffer, 4x, (Cat. # FB31010)

*pH should be between 7.7 and 7.8 at 25° C.

Ingredient MW Molarity Qty/Liter

Glycerol (40%) - - 400 g

Ficoll-400 (4%) - - 40 g

Triethanol amine, pH7.6 149.2 0.8M 120.0

6 N HCL 36.46 - 93.0 g

Lithium Dodecyl Sulfate (4%) - - 40 g

EDTA Di-Sodium 372.2 2mM 7.44 g

Brilliant Blue G250 (0.025%) - - 0.25g

Phenol Red - - 0.25 g

Ultrapure water (ll to) - - 1000 ml

Standard DNA/Native Running Buffer, 20X, (Cat. # GB61500)

*pH should be between 8.35 and 8.45 at 25° C.

Ingredient MW Molarity Qty/Liter

Tricine (free acid) 179.17 0.8M 143.4 g

Tris (free base) 121.14 1.2M 145.2 g

Ultrapure water (ll to) - - 1000ml

DNA/Native Sample Buffer, 4x, (Cat. # GB33002)

* pH should be 7.6 at 25° C.

Ingredient MW Molarity Qty/Liter

Glycerol (40%) - - 400 g

Ficoll-400 (4%) - - 40 g

Triethanol amine, pH7.6 149.2 0.8M 120.0

6 N HCL 36.46 - 93.0 g

EDTA Di-Sodium 372.2 2mM 7.44 g

Brilliant Blue G250 (0.025%) - - 0.25g

Phenol Red - - 0.25 g

Ultrapure water (ll to) - - 1000 ml

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Cbs-scientific QNX-700 Manuel d'utilisateur Page 20