Cbs-scientific DCX-800 Manuel d'utilisateur

Wide Dual Mini-VerticalsMini-Vertical Slab Gel /Blotting SystemDCX-800C, cooledDCX-800INSTRUCTION MANUAL

10Wide Dual Mini-Vertical Instructions 9-30-133.2 Running the Gel1. Place stirring bar into bottom of reservoir in stirring corral (as shown in gure

11www.cbsscientic.commatching colors3.3 Removing the Gel1. Turn the power supply off and disconnect the leads from the power supply. Remove the safe

12Wide Dual Mini-Vertical Instructions 9-30-134.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method (Not using Pre-cast gels)1. Place all c

13www.cbsscientic.com4.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method (Not using Pre-cast gels)1. Place all components in an authoriz

14Wide Dual Mini-Vertical Instructions 9-30-13SECTION 4 Alternate Protocol for Slab Gel Electrophoresis. (Not using Pre-cast gels)4.2 Preparing the

15www.cbsscientic.comSECTION 5 Instructions for Western Blotting5.1 Preparing the Unit for Blotting1. Remove safety cover from the assembled unit by

16Wide Dual Mini-Vertical Instructions 9-30-134. Insert blotting casettes into core making sure that red side faces outward. See diagram 5.5. Close do

17www.cbsscientic.com4. Insert blotting casettes into core making sure that red side faces outward. See diagram 5.5. Close doors and re-latch by pres

18Wide Dual Mini-Vertical Instructions 9-30-136.2 Electro-Blotting6.3 References1. Hames, B.D. (ed.) (1998). Gel Electrophoresis of Proteins. A Pra

19www.cbsscientic.com6.4 Recommended ClearPAGE™ Buffer FormulationsAs an alternative to the ClearPAGE buffers available for purchase, these formul

2Wide Dual Mini-Vertical Instructions 9-30-13TABLE OF CONTENTS Important User Information . .

20Wide Dual Mini-Vertical Instructions 9-30-136.4 Recommended ClearPAGE™ Buffer Formulations, con’t.LDS Sample Buffer, 4x, (Cat. # FB31010) *pH

21www.cbsscientic.comSECTION 7Maintenance of Equipment7.1 Care and Handling The plastic components of the Wide Dual Mini-Vertical Electrophoresis Sy

22Wide Dual Mini-Vertical Instructions 9-30-13SECTION 8Ordering InformationCOMBS: See page 6.Wide Dual Mini-Vertical Ordering Information CAT. # DES

23www.cbsscientic.comClearPAGE Precast Gel Selection Chart & Reagent Ordering information CAT. # DESCRIPTIONClearPAGE™ BUFFERS, STAI

CONTACT INFORMATIONOnline Ordering www.cbsscientic.comTelephone Local and International: 858-755-4959 Toll Free: 800-243-4959Sales E-mail Address

3www.cbsscientic.comIMPORTANT USER INFORMATIONThis Instruction Manual will explain how to use this product safely and effectively. Please read and c

4Wide Dual Mini-Vertical Instructions 9-30-13FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURSLe présent manuel d’utilisation explique la

5www.cbsscientic.comSECTION 1General Information 1.1 Introduction and FeaturesC.B.S. Scientic offers the Wide Dual Mini-Verticals are for performin

6Wide Dual Mini-Vertical Instructions 9-30-131.3 Comb Specications for Hand Cast Gels1.4 SafetyPower to the Wide Dual Mini-Vertical Electrophores

7www.cbsscientic.comSafety CoverCoreTubing adaptors(DCX-800C only)SECTION 2 Description of Parts2.1 Unpacking and Components Please verify that y

8Wide Dual Mini-Vertical Instructions 9-30-13SECTION 3 Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels3.1 Preparing the Electrophoresi

9www.cbsscientic.com45. Slide pre-cast gel cassette or plate set(s) into the core assembly with the notched plate facing in towards the upper buffer