Cbs-scientific DH Series Manuel d'utilisateur

Dedicated Height Nucleic Acid Sequencing SystemsINSTRUCTION MANUAL Single Unit Dual UnitDH-400-20 DDH-400-20DH-500-20 DDH-500-2

C.B.S.  Scientific 10 Dedicated Height Sequencer 3.1 Unit Assembly for Single Dedicated Height Sequencer-continued B. Installation of reservoirs

C.B.S.  Scientific 11 Dedicated Height Sequencer 3.3 Glass Plate Preparation 1. Select a pair of glass plates (if using a notched set, one n

C.B.S.  Scientific 12 Dedicated Height Sequencer 3.4 Methods for sealing glass plate sandwich (A) Gel Slider, ( B) Capillary Gel Casting o

C.B.S.  Scientific 13 Dedicated Height Sequencer 3.4 Methods for sealing glass plate sandwich - continued (A) Gel Slider, ( B) Capillary G

C.B.S.  Scientific 14 Dedicated Height Sequencer 3.5 Pouring the Gel 1. Always wear gloves, eye protection and protective clothing. 2. Pre

C.B.S.  Scientific 15 Dedicated Height Sequencer 6. Before the acrylamide polymerizes, insert a comb into the top of the gel. The available

C.B.S.  Scientific 16 Dedicated Height Sequencer Section 4 Electrophoresis 4.1 Pre-electrophoresis 1. Remove the gel sealing tape from the

C.B.S.  Scientific 17 Dedicated Height Sequencer 4. Verify that the upper buffer reservoir drain is secure. For the single 20cm wide sequenc

C.B.S.  Scientific 18 Dedicated Height Sequencer 4.3 Electrophoresis 1. After loading the samples, close the reservoir safety cover. Connec

C.B.S.  Scientific 19 Dedicated Height Sequencer 4.4 Post-Electrophoresis – Gel Processing A. Removal of sequencing plate from gel unit.

C.B.S.  Scientific 2 Dedicated Height Sequencer TABLE OF CONTENTS Page Important User Information 3-4 Section 1 General Information

C.B.S.  Scientific 20 Dedicated Height Sequencer B. Transfer of gel onto blotting paper-continued 2. Place a sheet of pre-cut (slightly l

C.B.S.  Scientific 21 Dedicated Height Sequencer 4.5 Recommended Buffers and Reagents Pre-mixed acrylamide stock solutions are the method

C.B.S.  Scientific 22 Dedicated Height Sequencer 4.6 References and Suggested Reference Literature DNA SEQUENCING PROTOCOLS in Methods in

C.B.S.  Scientific 23 Dedicated Height Sequencer Section 5 Tables: Power Settings, Troubleshooting Guide, Comb Volumes 5.1 Recommended

C.B.S.  Scientific 24 Dedicated Height Sequencer 5.2 Troubleshooting Guide Problem/Condition Cause & Solution ELECTRICAL  'No Loa

C.B.S.  Scientific 25 Dedicated Height Sequencer 5.3 Comb/Well Volumes Sequencing Combs for 20cm wide units Note: To calculate sample well

C.B.S.  Scientific 26 Dedicated Height Sequencer 46 well=5.92mm 62 well=4.50mm 68 well=4.02mm 94 well=3.00mm 5.3 Comb/

C.B.S.  Scientific 27 Dedicated Height Sequencer 5.3 Comb/Well Maximum Loading Volumes-continued Preparative Combs for 20cm wide units N

C.B.S.  Scientific 28 Dedicated Height Sequencer Section 6 Maintenance of Equipment 6.1 Care and Handling The components of the Sequencer ar

C.B.S.  Scientific 29 Dedicated Height Sequencer Section 7 Equipment and Accessories Dedicated Height Sequencers Cat.# Item Si

C.B.S.  Scientific 3 Dedicated Height Sequencer IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely an

C.B.S.  Scientific 30 Dedicated Height Sequencer SEQUENCING ACCESSORIES RECTANGULAR COMBS (POLYCARBONATE) 20CM WIDE UNITS 33CM WIDE UNIT

C.B.S.  Scientific 31 Dedicated Height Sequencer PREPARATIVE RECTANGULAR COMBS (TEFLON) 20CM WIDE UNITS Cat.# Comb Dimensions Cat.#

C.B.S.  Scientific 32 Dedicated Height Sequencer LOW FLUORESCENCE GLASS PLATE SETS 20CM WIDE UNITS 33CM WIDE UNITS Cat.# Plate Dimensi

C.B.S.  Scientific 33 Dedicated Height Sequencer MICROTROUGH CASTING COMBS 20CM WIDE UNITS 33CM WIDE UNITS Cat.# Thickness x # trough

C.B.S.  Scientific 34 Dedicated Height Sequencer MYLARSPACER PAIRS FOR 20CM & 33CM WIDE UNITS Cat.# Spacer Dimensions Cat.# Space

C.B.S.  Scientific 35 Dedicated Height Sequencer WEDGE SPACER SETS FOR 20CM AND 33CM WIDE UNITS EXPONENTIAL 1:3 RATIO EXPONENTIAL 1:5 RATIO

C.B.S.  Scientific 36 Dedicated Height Sequencer ULTRATHIN MYLAR ACCESSORIES FOR 20CM & 33CM WIDE UNITS 20CM WIDE UNITS 33CM WIDE

C.B.S.  Scientific 37 Dedicated Height Sequencer SLIDING GEL CASTERS Cat.# Item GSL-1001 Casts glass plates 20cm-38cm (w) x 22

NOTES

NOTES

C.B.S.  Scientific 4 Dedicated Height Sequencer FRANÇAIS INFORMATION IMPORTANTE À L'USAGE DES UTILISATEURS Le présent manuel d'utilis

Telephone:Local or International858-755-4959Toll Free: 858-755-0733Fax: 858-755-0733Check out our new and improved online ordering!www.cbsscientific.co

C.B.S.  Scientific 5 Dedicated Height Sequencer General Information 1.1 Introduction The C.B.S. Scientific Dedicated Height Nucleic Acid Sequenc

C.B.S.  Scientific 6 Dedicated Height Sequencer 1.3 Safety Power to the Sequencing unit must be supplied by an external DC voltage power supply t

C.B.S.  Scientific 7 Dedicated Height Sequencer Section 2 Description of Parts 2.1 Unpacking- Dedicated Height Sequencer Please verify that

C.B.S.  Scientific 8 Dedicated Height Sequencer 2.2 Unpacking- Dual Dedicated Height Sequencer Please verify that your sequencing unit comes c

C.B.S.  Scientific 9 Dedicated Height Sequencer Assembly 3.1 Unit Assembly for Single Dedicated Height Sequencer Place the Sequencer in a labor