Cbs-scientific DCX-700 Manuel d'utilisateur

Dual Cool Electrophoresis SystemMini-Vertical Slab Gel /Blotting SystemDCX-700INSTRUCTION MANUAL

10Dual Cool DCX-700 Instructions 9-30-133.2 Running the Gel1. Place core assembly into lower reservoir. The anode (red) and cathode (black) electrode

11www.cbsscientic.commatching colors894. Attach safety cover and turn on magnetic stirrer. The closed unit ready for power is shown in gure 8.5. Co

12Dual Cool DCX-700 Instructions 9-30-134.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method (Not using Pre-cast gels)1. Place all compone

13www.cbsscientic.com4.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method (Not using Pre-cast gels)1. Place all components in an authoriz

14Dual Cool DCX-700 Instructions 9-30-13SECTION 4 Alternate Protocol for Slab Gel Electrophoresis. (Not using Pre-cast gels)4.2 Preparing the Electr

15www.cbsscientic.com1SECTION 5 Instructions for Western Blotting5.1 Preparing the Unit for Blotting1. Remove safety cover from the assembled unit b

16Dual Cool DCX-700 Instructions 9-30-134. Insert blotting casettes into core making sure that red side faces outward. See diagram 5.5. Close doors an

17www.cbsscientic.com4. Insert blotting casettes into core making sure that red side faces outward. See diagram 5.5. Close doors and re-latch by pres

18Dual Cool DCX-700 Instructions 9-30-136.2 Electro-Blotting6.3 References1. Hames, B.D. (ed.) (1998). Gel Electrophoresis of Proteins. A Practical

19www.cbsscientic.comStandard SDS Running Buffer, 20X for Reduced Samples (Cat. # FB60500)* pH should be between 8.4 and 8.5 at 25° C.Ingredient MW

2Dual Cool DCX-700 Instructions 9-30-13TABLE OF CONTENTS Important User Information . . . .

20Dual Cool DCX-700 Instructions 9-30-136.4 Recommended ClearPAGE™ Buffer Formulations, con’t.LDS Sample Buffer, 4x, (Cat. # FB31010) *pH shoul

21www.cbsscientic.comSECTION 7Maintenance of Equipment7.1 Care and Handling The plastic components of the Dual Cool Electrophoresis Systems are fabr

22Dual Cool DCX-700 Instructions 9-30-13SECTION 8Ordering Information CAT. # DESCRIPTIONDUAL COOL MINI-VERTICALS - 10CM(W) X 10CM(H)DCX-700Dual C

23www.cbsscientic.comClearPAGE SDS gels, packages of 10 - Cat. #’sAcrylamide %Gel Size (cm) W x H1 wellCat. #2D well trough +1 marker laneCat. #12 we

CONTACT INFORMATIONOnline Ordering www.cbsscientic.comTelephone Local and International: 858-755-4959 Toll Free: 800-243-4959Sales E-mail Address

3www.cbsscientic.comIMPORTANT USER INFORMATIONThis Instruction Manual will explain how to use this product safely and effectively. Please read and c

4Dual Cool DCX-700 Instructions 9-30-13FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURSLe présent manuel d’utilisation explique la manière

5www.cbsscientic.comSECTION 1General Information 1.1 Introduction and FeaturesC.B.S. Scientic offers the Dual Cool Electrophoresis System for perfo

6Dual Cool DCX-700 Instructions 9-30-131.3 Comb Specications for Hand Cast Gels1.4 SafetyPower to the Dual Cool Electrophoresis System is to be s

7www.cbsscientic.comSafety CoverCoreReservoirFreezer BlockSECTION 2 Description of Parts2.1 Unpacking and Components Please verify that your unit

8Dual Cool DCX-700 Instructions 9-30-13SECTION 3 Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels3.1 Preparing the Electrophoresis Unit

9www.cbsscientic.com45. Slide pre-cast gel cassette or plate set(s) into the core assembly with the notched plate facing in towards the upper buffer